2018
Almeida C.M., Simões I. Cardoon-based rennets for cheese production Appl Microbiol Biotechnol (2018) https://doi.org/10.1007/s00253-018-9032-3
Almeida, C., Simões, I., Faro C., US Patent No. 9,890,372, granted 13 February 2018 – Aspartic Proteases: Methods and composition, thereof, relate to aspartic proteases, and particularly to aspartic proteases for plants. Disclosed are modified plant aspartic proteases, and methods for their manufacture, and uses thereof. Particularly contemplated are the uses of aspartic proteases in inducing skin desquamation.
2017
Almeida, C.M., Manso, J.A., Figueiredo, A.C. et al. Functional and structural characterization of synthetic cardosin B-derived rennet Appl Microbiol Biotechnol (2017). https://doi.org/10.1007/s00253-017-8445-8
2016
Curto P., Simões I., Riley S.P., Martinez J.J. Differences in Intracellular Fate of Two Spotted Fever Group Rickettsia in Macrophage-Like Cells Front. Cell. Infect. Microbiol. 2016 6:80. DOI: 10.3389/fcimb.2016.00080.
Leal AR, Cruz R, Bur D, Huesgen PF, Faro R, Manadas B, Wlodawer A, Faro C, Simões I. Enzymatic properties, evidence for in vivo expression, and intracellular localization of shewasin D, the pepsin homolog from Shewanella denitrificans. Sci Rep. 2016 Mar 31;6:23869. doi: 10.1038/srep23869. PubMed PMID: 27029611; PubMed Central PMCID: PMC4814920.
Dias DM, Furtado J, Wasielewski E, Cruz R, Costello B, Cole L, Faria TQ, Baaske P, Brito RM, Ciulli A, Simões I, Macedo-Ribeiro S, Faro C, Geraldes CF, Castanheira P. Biophysical characterization of laforin-carbohydrate interaction. Biochem J. 2016 Feb 1;473(3):335-45. doi: 10.1042/BJ20141555. Epub 2015 Nov 17. PubMed PMID: 26578817.
2015
Li M, Gustchina A, Cruz R, Simões M, Curto P, Martinez J, Faro C, Simões I, Wlodawer A. Structure of RC1339/APRc from Rickettsia conorii, a retropepsin-like aspartic protease. Acta Crystallogr D Biol Crystallogr. 2015 Oct;71(Pt 10):2109-18. doi: 10.1107/S1399004715013905. Epub 2015 Sep 30. PubMed PMID: 26457434; PubMed Central PMCID: PMC4601372.
Almeida CM, Gomes D, Faro C, Simões I. Engineering a cardosin B-derived rennet for sheep and goat cheese manufacture. Appl Microbiol Biotechnol. 2015 Jan;99(1):269-81. doi: 10.1007/s00253-014-5902-5. Epub 2014 Jul 2. PubMed PMID: 24986551.
2014
Cruz R, Huesgen P, Riley SP, Wlodawer A, Faro C, Overall CM, Martinez JJ, Simões I. RC1339/APRc from Rickettsia conorii is a novel aspartic protease with properties of retropepsin-like enzymes. PLoS Pathog. 2014 Aug 21;10(8):e1004324. doi: 10.1371/journal.ppat.1004324. eCollection 2014 Aug. PubMed PMID: 25144529; PubMed Central PMCID: PMC4140852.
Curto P, Lufrano D, Pinto C, Custódio V, Gomes AC, Trejo SA, Bakás L, Vairo-Cavalli S, Faro C, Simões I. Establishing the yeast Kluyveromyces lactis as an expression host for production of the saposin-like domain of the aspartic protease cirsin. Appl Environ Microbiol. 2014 Jan;80(1):86-96. doi: 10.1128/AEM.03151-13. Epub 2013 Oct 11. PubMed PMID: 24123748; PubMed Central PMCID: PMC3910990.
2013
Resende R, Marques SC, Ferreiro E, Simões I, Oliveira CR, Pereira CM. Effect of α-synuclein on amyloid β-induced toxicity: relevance to Lewy body variant of Alzheimer disease. Neurochem Res. 2013 Apr;38(4):797-806. doi: 10.1007/s11064-013-0982-7. Epub 2013 Feb 7. PubMed PMID: 23389658.
2012
Lufrano D, Faro R, Castanheira P, Parisi G, Veríssimo P, Vairo-Cavalli S, Simões I, Faro C. Molecular cloning and characterization of procirsin, an active aspartic protease precursor from Cirsium vulgare (Asteraceae). Phytochemistry. 2012 Sep;81:7-18. doi: 10.1016/j.phytochem.2012.05.028. Epub 2012 Jun 21. PubMed PMID: 22727116.
Almeida CM, Pereira C, da Costa DS, Pereira S, Pissarra J, Simões I, Faro C. Chlapsin, a chloroplastidial aspartic proteinase from the green algae Chlamydomonas reinhardtii. Planta. 2012 Jul;236(1):283-96. doi: 10.1007/s00425-012-1605-2. Epub 2012 Feb 19. PubMed PMID: 22349731.
2011
Simões I, Faro R, Bur D, Kay J, Faro C. Shewasin A, an active pepsin homolog from the bacterium Shewanella amazonensis.FEBS J. 2011 Sep;278(17):3177-86. doi: 10.1111/j.1742-4658.2011.08243.x. Epub 2011 Aug 11. PubMed PMID: 21749650.